Coding

Part:BBa_K3008000:Design

Designed by: Shayan Vahdani   Group: iGEM19_UCSC   (2019-08-23)


rvLEAM


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 19
    Illegal NgoMIV site found at 163
    Illegal NgoMIV site found at 349
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Induced a silent mutation by changing GGC to GGT (glycine) to remove a NotI restriction site (position 870). The sequence was codon optimized for E. coli using a self-written program from the Silver lab at Harvard.


Source

This protein comes from the genome of tardigrade species, Ramazzottius varieornatus. It is found in the mitochondria of various species of tardigrades.

References